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Int J Oncol. 2014 Sep;45(3):1059-64. doi: 10.3892/ijo.2014.2507. Epub 2014 Jun 20.

Human β-defensin-3 inhibits migration of colon cancer cells via downregulation of metastasis-associated 1 family, member 2 expression.

Author information

1
First Department of Internal Medicine, Mie University School of Medicine, Tsu, Japan.
2
Department of Gastroenterology, Mie University School of Medicine, Tsu, Japan.
3
Department of Molecular and Laboratory Medicine, Mie University School of Medicine, Tsu, Japan.

Abstract

The innate immune system plays an important role as the first line of defense against many types of microbes. Accumulating reports suggest that human β-defensins (hBDs) are expressed by and have certain roles in some cancer cells. In this study, we investigated the roles of hBD-3 in colon cancer cells. The expression of hBD-3 was examined by reverse transcriptase-polymerase chain reaction analysis of colon cancer cell lines and immunohistochemical staining of colon cancer tissues. The effect of hBD-3 on proliferation of colon cancer was assessed using the MTT assay and a real-time cell analyzer, and the effect of hBD-3 on the migration of colon cancer cells was also examined. The results showed that hBD-3 is not expressed in colon cancer cells but is produced by tumor-infiltrating monocytes. Migration of colon cancer cells was significantly inhibited by hBD-3 in a dose-dependent manner, although proliferation of colon cancer cells was not affected by administration of hBD-3. Moreover, reduced expression of metastasis-associated 1 family, member 2 (MTA2) mRNA in colon cancer cells was associated with exposure to hBD-3. In conclusion, progression of colon cancer was inhibited by hBD-3 in a paracrine fashion. Therefore, hBD-3 may be a potent new agent for treating colon cancer.

PMID:
24969834
DOI:
10.3892/ijo.2014.2507
[Indexed for MEDLINE]
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