Format

Send to

Choose Destination
See comment in PubMed Commons below
Dev Comp Immunol. 2014 Nov;47(1):17-24. doi: 10.1016/j.dci.2014.06.005. Epub 2014 Jun 23.

Oligochitosan stimulated phagocytic activity of macrophages from blunt snout bream (Megalobrama amblycephala) associated with respiratory burst coupled with nitric oxide production.

Author information

1
Department of Aquatic Animal Medicines, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
2
Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, China; Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, Hubei 430070, China.
3
Division of Fish Pathology, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, Hubei 430223, China.
4
Department of Aquatic Animal Medicines, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, China. Electronic address: liuxl@mail.hzau.edu.cn.
5
Department of Aquatic Animal Medicines, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, China; Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, Hubei 430070, China; State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. Electronic address: linli@mail.hzau.edu.cn.

Abstract

The immunostimulating effects of oligochitosan have been proven in several fish, however, the mechanisms underlying the stimulation are not characterized. In the present study, the effects of oligochitosan were investigated using macrophages isolated from blunt snout bream (Megalobrama amblycephala). The results showed that the phagocytic activity of the macrophages was enhanced by the addition of oligochitosan in vitro and in vivo. The two of the most important antimicrobial pathways of macrophages, NADPH oxidase and iNOS pathways were included for further studies. The amounts of superoxide anion and the mRNAs of the five subunits of NADPH oxidase genes were significantly enhanced in the oligochitosan-treated macrophages and macrophages isolated from fish fed with feed containing oligochitosan. In addition, the NO production, iNOS activity and iNOS gene expression were all significantly increased in the presence of oligochitosan. Furthermore, the mRNA levels of the TNF-α and IL-1β were also significantly increased in the macrophages derived from fish fed with oligochitosan. In conclusion, the stimulation effects of oligochitosan on the phagocytic activity of the fish macrophages were associated with respiratory burst coupled with nitric oxide production.

KEYWORDS:

Macrophage; Megalobrama amblycephala; Nitric oxide production; Oligochitosan; Phagocytosis; Respiratory burst

PMID:
24968077
DOI:
10.1016/j.dci.2014.06.005
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center