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PLoS One. 2014 Jun 26;9(6):e100791. doi: 10.1371/journal.pone.0100791. eCollection 2014.

Hijacking of host cellular functions by an intracellular parasite, the microsporidian Anncaliia algerae.

Author information

1
Clermont Université, Université Blaise Pascal, Laboratoire Microorganismes: Génome et Environnement, Clermont-Ferrand, France; CNRS, UMR 6023, LMGE, Aubière, France.
2
Functional Proteomics Platform. UMR CNRS 5203, Montpellier, France.
3
Functional Proteomics Platform. UMS CNRS 3426, Montpellier, France.
4
INSERM, UMR1090 TAGC, Marseille, Marseille, France; Aix-Marseille Université, UMR1090 TAGC, Marseille, France; CNRS, Marseille, France.
5
INSERM, UMR1090 TAGC, Marseille, Marseille, France; Aix-Marseille Université, UMR1090 TAGC, Marseille, France.
6
Clermont Université, Université d'Auvergne, I.U.T., UFR Pharmacie, Clermont-Ferrand, France; Clermont Université, Université d'Auvergne, EA 4678, Conception, Ingénierie et Développement de l'Aliment et du Médicament, Clermont-Ferrand, France.
7
Université de Lyon, Université Lyon 1, CNRS, UMR5558, Laboratoire de Biométrie et Biologie Evolutive, Villeurbanne, France.

Abstract

Intracellular pathogens including bacteria, viruses and protozoa hijack host cell functions to access nutrients and to bypass cellular defenses and immune responses. These strategies have been acquired through selective pressure and allowed pathogens to reach an appropriate cellular niche for their survival and growth. To get new insights on how parasites hijack host cellular functions, we developed a SILAC (Stable Isotope Labeling by Amino Acids in Cell culture) quantitative proteomics workflow. Our study focused on deciphering the cross-talk in a host-parasite association, involving human foreskin fibroblasts (HFF) and the microsporidia Anncaliia algerae, a fungus related parasite with an obligate intracellular lifestyle and a strong host dependency. The host-parasite cross-talk was analyzed at five post-infection times 1, 6, 12 and 24 hours post-infection (hpi) and 8 days post-infection (dpi). A significant up-regulation of four interferon-induced proteins with tetratricopeptide repeats IFIT1, IFIT2, IFIT3 and MX1 was observed at 8 dpi suggesting a type 1 interferon (IFN) host response. Quantitative alteration of host proteins involved in biological functions such as signaling (STAT1, Ras) and reduction of the translation activity (EIF3) confirmed a host type 1 IFN response. Interestingly, the SILAC approach also allowed the detection of 148 A. algerae proteins during the kinetics of infection. Among these proteins many are involved in parasite proliferation, and an over-representation of putative secreted effectors proteins was observed. Finally our survey also suggests that A. algerae could use a transposable element as a lure strategy to escape the host innate immune system.

PMID:
24967735
PMCID:
PMC4072689
DOI:
10.1371/journal.pone.0100791
[Indexed for MEDLINE]
Free PMC Article

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