Format

Send to

Choose Destination
Iran J Basic Med Sci. 2014 May;17(5):378-83.

Genetic properties of blaCTX-M and blaPER β-lactamase genes in clinical isolates of Enterobacteriaceae by polymerase chain reaction.

Author information

1
Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
2
Department of Biology, Science and Research Branch, Islamic Azad University, Fars, Iran.
3
Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
4
Medical Genetic Research Center, School of Medical Science, Mashhad, Iran.

Abstract

OBJECTIVES:

blaCTX-M and blaPER are two genes that encode class A extended-spectrum β-lactamases (ESBLs) and can be responsible for therapeutic problems. This study was carried out to evaluate the molecular properties of these genes in clinical isolates of Enterobacteriaceae by polymerase chain reaction (PCR), restriction digestion and sequencing.

MATERIALS AND METHODS:

During six months, starting from January 2012, one hundred clinical isolates of Enterobacteriaceae were collected from urinary samples. The ESBL-producing isolates were detected by phenotypic confirmation test. After plasmid extraction, blaPER and blaCTX-M genes were detected using PCR by specific primers. The blaCTX-M PCR products were digested with Taq1, and two of the blaCTX-M genes were sequenced.

RESULTS:

Phenotypic tests showed that 27 (27%) isolates were ESBL producers with the highest frequency for Klebsiella pneumoniae (47.4%) and Escherichia coli (17.9%). Twenty six (26%) of Enterobacteriaceae isolates harbored the blaCTX-M gene, and none of them had blaPER . The restriction analysis of PCR products showed that all blaCTX-M amplified products had the same patterns. Both sequenced bacteria were CTX-M-15 type ESBL carriers.

CONCLUSION:

The results of this study showed the blaCTX-M-15 gene in Enterobacteriaceae isolates for the first time in Mashhad, Iran. High degrees of associated resistance to co-trimoxazole and gentamicin were found in ESBL producers. Therefore, an integrated and regular management of antibiotic prescription need to be trained in our society.

KEYWORDS:

CTX-M-15; Enterobacteriaceae; Extended-spectrum beta-lactamase; PER

PMID:
24967067
PMCID:
PMC4069837

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center