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Cutan Ocul Toxicol. 2015;34(2):101-6. doi: 10.3109/15569527.2014.910802. Epub 2014 Jun 25.

The radioprotective effect of Nigella sativa on nitrosative stress in lens tissue in radiation-induced cataract in rat.

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1
Department of Medical Biochemistry .

Abstract

OBJECTIVE:

The aim of this study was to investigate the antioxidant and radioprotective effects of Nigella sativa oil (NSO) and thymoquinone (TQ) against ionizing radiation-induced cataracts in lens after total cranium irradiation (IR) of rats with a single dose of 5 gray (Gy).

MATERIALS AND METHODS:

Seventy-four Sprague-Dawley rats were used for the experiment. The rats were randomly divided into six groups. Group A received total cranium IR plus NSO (1 g kg(-1) d(-1)) orally through an orogastric tube. Group B received total cranium IR plus TQ (50 mgkg(-1) d(-1)) daily by intraperitoneal injection. Group C received 5 Gy of gamma IR as a single dose to total cranium plus 1 ml saline. Group D1 just received 1 ml saline. Group D2 just received dimethyl sulfoxide. Group D3 did not receive anything.

RESULTS:

At the end of the 10th d, cataract developed in 80% of the rats in IR group only. After IR, cataract rate dropped to 20% and 50% in groups which were treated with NSO and TQ, respectively, and was limited at grades 1 and 2. Nitric oxide synthase activity, nitric oxide and peroxynitrite levels in the radiotherapy group were higher than those of all other groups.

CONCLUSIONS:

The results implicate a major role for NSO and TQ in preventing cataractogenesis in ionizing radiation-induced cataracts in the lenses of rats, wherein NSO were found to be more potent.

KEYWORDS:

Cataract; Nigella sativa; free radical; irradiation; nitric oxide; nitrosative stress

PMID:
24964170
DOI:
10.3109/15569527.2014.910802
[Indexed for MEDLINE]
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