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Biotechnol Res Int. 2014;2014:705068. doi: 10.1155/2014/705068. Epub 2014 May 22.

Characterization of modified magnetite nanoparticles for albumin immobilization.

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Department of Chemistry, University of Isfahan, Isfahan 81746-73441, Iran ; Department of Biotechnology, Faculty of New Science and Technology, University of Isfahan, Isfahan 81746-73441, Iran.
Department of Molecular and Cell Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
Department of Chemistry, University of Isfahan, Isfahan 81746-73441, Iran.


Magnetite Fe3O4 nanoparticles (NPs) were prepared by chemical coprecipitation method. Silica-coated magnetite NPs were prepared by sol-gel reaction, subsequently coated with 3-aminopropyltriethoxysilane (APTES) via silanization reaction, and then were activated with 2,4,6-trichloro-1,3,5-triazine (TCT) and covalently immobilized with bovine serum albumin (BSA). The size and structure of the particles were characterized by transmission electron microscopy (TEM), X-ray powder diffraction (XRD), and dynamic light scattering (DLS) techniques. The immobilization was confirmed by Fourier transform infrared spectroscopy (FT-IR). XRD analysis showed that the binding process has not done any phase change to Fe3O4. The immobilization time for this process was 4 h and the amount of immobilized BSA for the initial value of 1.05 mg BSA was about 120 mg/gr nanoparticles. Also, the influences of three different buffer solutions and ionic strength on covalent immobilization were evaluated.

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