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Evid Based Complement Alternat Med. 2014;2014:158018. doi: 10.1155/2014/158018. Epub 2014 May 14.

Effect of Achyranthes bidentata Blume on 3T3-L1 Adipogenesis and Rats Fed with a High-Fat Diet.

Author information

1
Department of East-West Medicine, Graduate School, Kyung Hee University, Seoul, Republic of Korea.
2
Department of Medical Science, Graduate School of East-West Medical Science, Kyung Hee University, Yongin, Republic of Korea.
3
Department of East-West Medicine, Graduate School, Kyung Hee University, Seoul, Republic of Korea ; Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Republic of Korea.
4
Acupuncture & Meridian Science Research Center, Kyung Hee University, Seoul, Republic of Korea ; Division of Homeostatic Development, National Institute for Physiological Sciences, Okazaki, Japan.
5
Department of Physiology, College of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea.
6
Department of Oriental Pediatrics, Kyung Hee Medical Center, Kyung Hee University, Seoul, Republic of Korea.
7
Department of Family Medicine, CHA University School of Medicine, Seoul, Republic of Korea.
8
Department of East-West Medicine, Graduate School, Kyung Hee University, Seoul, Republic of Korea ; Department of Integrative Medicine, CHA University School of Medicine, 442 Dosan-daero, Gangnam-gu, Seoul 135-948, Republic of Korea.

Abstract

The present study investigated the antiobesity effect of Achyranthes bidentata Blume root water extract in a 3T3-L1 adipocyte differentiation model and rats fed with a high-fat diet. To investigate the effect of Achyranthes bidentata Blume on adipogenesis in vitro, differentiating 3T3-L1 cells in adipocyte-induction media were treated every two days with Achyranthes bidentata Blume at various concentrations (1 to 25  μ g/mL) for eight days. We found that Achyranthes bidentata Blume root inhibited 3T3-L1 adipocyte differentiation without affecting cell viability, and Western blot analysis revealed that phospho-Akt expression was markedly decreased, whereas there was no significant change in perilipin expression. Furthermore, administration of Achyranthes bidentata Blume root (0.5 g/kg body weight for six weeks) to rats fed with a high-fat diet significantly reduced body weight gain without affecting food intake, and the level of triglyceride was significantly decreased when compared to those in rats fed with only a high-fat diet. These results suggest that Achyranthes bidentata Blume root water extract could have a beneficial effect on inhibition of adipogenesis and controlling body weight in rats fed with a high-fat diet.

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