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Clin Interv Aging. 2014 Jun 12;9:939-44. doi: 10.2147/CIA.S60330. eCollection 2014.

Concentration of cytokines in patients with osteoarthritis of the knee and fibromyalgia.

Author information

1
Clinical Research Center, Institute of Physical Medicine and Rehabilitation, University of São Paulo Medical School, São Paulo, Brazil.
2
Division of Physical Medicine, Center for Acupuncture, Institute of Orthopedics and Traumatology, University of São Paulo, São Paulo, Brazil ; Department of Pathology, University of São Paulo, São Paulo, Brazil.
3
Department of Pathology, University of São Paulo, São Paulo, Brazil.
4
Laboratory of Medical Investigation LIM-52-HCFMUSP, Institute of Tropical Medicine, University of São Paulo, São Paulo, Brazil.
5
Tropical Laboratory of Hematology/Medical Research Laboratory-LIM 52, Institute of Tropical Medicine, University of São Paulo, São Paulo, Brazil.
6
Clinical Research Center, Institute of Physical Medicine and Rehabilitation, University of São Paulo Medical School, São Paulo, Brazil ; Program of Master in Health Promotion, São Paulo Adventist University Center, São Paulo, Brazil.
7
Clinical Research Center, Institute of Physical Medicine and Rehabilitation, University of São Paulo Medical School, São Paulo, Brazil ; Department of Legal Medicine, Medical Ethics, Social and Labor Medicine - Medicine School, University of São Paulo, São Paulo, Brazil.

Abstract

INTRODUCTION:

Fibromyalgia and osteoarthritis may present a relationship with the concentration of cytokines. The aim of this study was to compare the serum concentrations of IL-12p70, tumor necrosis factor, IL-10, IL-6, IL-1β, and IL-8 in patients with knee osteoarthritis and fibromyalgia.

MATERIALS AND METHODS:

The study included 53 women (71.2±7.6 years old) diagnosed with knee osteoarthritis with moderate-to-severe pain (visual analog scale >4) for at least 3 months. Sixty women (54.1±8.1 years old) diagnosed with fibromyalgia according to the American College of Rheumatology criteria and with moderate-to-severe pain (visual analog scale >4) also participated in this study. For the dosage of cytokines, blood was collected in the morning: 5 mL from the cubital vein. The material was centrifuged at 4°C, separated into 100 μL aliquots and stored at -80°C until processing. Serum concentrations of the studied cytokines were assessed using the BD Cytometric Bead Array method. Data were analyzed with Student's t-test and the Mann-Whitney U test.

RESULTS:

We found higher levels of IL-6, IL-10, and IL-1β in fibromyalgia patients. After adjustment of age as a covariate, there was no statistically significant difference in the concentration of any cytokine between fibromyalgia and knee osteoarthritis patients.

CONCLUSION:

Patients with knee osteoarthritis and fibromyalgia with the same duration and intensity of pain demonstrate similar concentrations of cytokines. Aging may play a role in cytokine profile, a finding not so extensively addressed in the literature and one that should be further investigated.

KEYWORDS:

aging; cytokines; fibromyalgia; osteoarthritis

PMID:
24959074
PMCID:
PMC4061171
DOI:
10.2147/CIA.S60330
[Indexed for MEDLINE]
Free PMC Article

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