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J Clin Microbiol. 2014 Sep;52(9):3237-43. doi: 10.1128/JCM.01258-14. Epub 2014 Jun 23.

Detection of chronic wasting disease in the lymph nodes of free-ranging cervids by real-time quaking-induced conversion.

Author information

1
Prion Research Center, Department of Microbiology, Immunology, and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA nicholas.j.haley@gmail.com.
2
School of Biological Sciences, University of Tasmania, Hobart, Tasmania, Australia.
3
Prion Research Center, Department of Microbiology, Immunology, and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA.
4
Department of Population Medicine and Diagnostic Sciences, Cornell University College of Veterinary Medicine, Ithaca, New York, USA.
5
Texas Parks and Wildlife Department, Austin, Texas, USA.
6
Nebraska Game and Parks Commission, Lincoln, Nebraska, USA.
7
University of Nebraska-Lincoln Veterinary Diagnostic Center, Lincoln, Nebraska, USA.
8
Colorado Parks and Wildlife, Fort Collins, Colorado, USA.
9
Division of Wildlife Resources, Illinois Department of Natural Resources, Springfield, Illinois, USA.

Abstract

Chronic wasting disease (CWD), a transmissible spongiform encephalopathy of deer, elk, and moose, is the only prion disease affecting free-ranging animals. Since the disease was first identified in northern Colorado and southern Wyoming in 1967, new epidemic foci of the disease have been identified in 20 additional states, as well as two Canadian provinces and the Republic of South Korea. Identification of CWD-affected animals currently requires postmortem analysis of brain or lymphoid tissues using immunohistochemistry (IHC) or an enzyme-linked immunosorbent assay (ELISA), with no practical way to evaluate potential strain types or to investigate the epidemiology of existing or novel foci of disease. Using a standardized real-time (RT)-quaking-induced conversion (QuIC) assay, a seeded amplification assay employing recombinant prion protein as a conversion substrate and thioflavin T (ThT) as an amyloid-binding fluorophore, we analyzed, in a blinded manner, 1,243 retropharyngeal lymph node samples from white-tailed deer, mule deer, and moose, collected in the field from areas with current or historic CWD endemicity. RT-QuIC results were then compared with those obtained by conventional IHC and ELISA, and amplification metrics using ThT and thioflavin S were examined in relation to the clinical history of the sampled deer. The results indicate that RT-QuIC is useful for both identifying CWD-infected animals and facilitating epidemiological studies in areas in which CWD is endemic or not endemic.

PMID:
24958799
PMCID:
PMC4313144
DOI:
10.1128/JCM.01258-14
[Indexed for MEDLINE]
Free PMC Article
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