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Am J Trop Med Hyg. 2014 Sep;91(3):589-97. doi: 10.4269/ajtmh.14-0146. Epub 2014 Jun 23.

Phylogeography of Rickettsia rickettsii genotypes associated with fatal Rocky Mountain spotted fever.

Author information

1
Infectious Diseases Pathology Branch and Rickettsial Zoonoses Branch, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Núcleo de Anatomica Patológia, Centro de Patologia do Instituto Adolfo Lutz, São Paulo, Brazil; Departamento de Vigilância em Saúde, Secretaria Municipal de Saúde de Campinas, São Paulo, Brazil cdp9@cdc.gov.
2
Infectious Diseases Pathology Branch and Rickettsial Zoonoses Branch, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia; Núcleo de Anatomica Patológia, Centro de Patologia do Instituto Adolfo Lutz, São Paulo, Brazil; Departamento de Vigilância em Saúde, Secretaria Municipal de Saúde de Campinas, São Paulo, Brazil.

Abstract

Rocky Mountain spotted fever (RMSF), a tick-borne zoonosis caused by Rickettsia rickettsii, is among the deadliest of all infectious diseases. To identify the distribution of various genotypes of R. rickettsii associated with fatal RMSF, we applied molecular typing methods to samples of DNA extracted from formalin-fixed, paraffin-embedded tissue specimens obtained at autopsy from 103 case-patients from seven countries who died of RMSF. Complete sequences of one or more intergenic regions were amplified from tissues of 30 (29%) case-patients and revealed a distribution of genotypes consisting of four distinct clades, including the Hlp clade, regarded previously as a non-pathogenic strain of R. rickettsii. Distinct phylogeographic patterns were identified when composite case-patient and reference strain data were mapped to the state and country of origin. The phylogeography of R. rickettsii is likely determined by ecological and environmental factors that exist independently of the distribution of a particular tick vector.

PMID:
24957541
PMCID:
PMC4155566
DOI:
10.4269/ajtmh.14-0146
[Indexed for MEDLINE]
Free PMC Article
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