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Chemosphere. 2014 Dec;117:47-52. doi: 10.1016/j.chemosphere.2014.05.069. Epub 2014 Jun 21.

Biodegradation of methyl parathion by whole cells of marine-derived fungi Aspergillus sydowii and Penicillium decaturense.

Author information

1
Laboratório de Química Orgânica e Biocatálise, Instituto de Química de São Carlos, Universidade de São Paulo, Av. João Dagnone, n° 1100, Ed. Química Ambiental, Jd. Santa Angelina, 13563-120 São Carlos, São Paulo, Brazil.
2
Laboratório de Ecologia e Microbiologia Aquática, Universidade Federal de São Carlos, Via Washington Luís, Km 235, 13565-905 São Carlos, São Paulo, Brazil.
3
Laboratório de Química Orgânica e Biocatálise, Instituto de Química de São Carlos, Universidade de São Paulo, Av. João Dagnone, n° 1100, Ed. Química Ambiental, Jd. Santa Angelina, 13563-120 São Carlos, São Paulo, Brazil. Electronic address: almporto@iqsc.usp.br.

Abstract

Seven marine fungi strains (Aspergillus sydowii CBMAI 934, A. sydowii CBMAI 935, A. sydowii CBMAI 1241, Penicillium decaturense CBMAI 1234, Penicillium raistrickii CBMAI 931, P. raistrickii CBMAI 1235, and Trichoderma sp. CBMAI 932) were screened by their growth in the presence of methyl parathion (MP) in a solid culture medium. The strains with best growth were A. sydowii CBMAI 935 and P. decaturense CBMAI 1234. Biodegradation reactions were performed in 10, 20 and 30d in a malt extract liquid medium containing commercial MP and whole cells of A. sydowii CBMAI 935 and P. decaturense CBMAI 1234. In 20d, A. sydowii CBMAI 935 was able to degrade all pesticide, whereas P. decaturense CBMAI 1234 promoted a complete degradation in 30d. A. sydowii CBMAI 935 and P. decaturense CBMAI 1234 could degrade the product of the MP enzymatic hydrolysis, p-nitrophenol, on average of 51 and 40% respectively. Both strains used MP as a sole source of carbon and provided satisfactory results. Metabolites detected in the medium showed that the presumable reaction pathway occurred through the activation of MP to its more toxic form, methyl paraoxon, which was further degraded to p-nitrophenol.

KEYWORDS:

Enzymatic hydrolysis; Marine-derived fungi; Organophosphate pesticides; p-Nitrophenol

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