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Mol Cell Endocrinol. 2014 Aug 5;393(1-2):99-108. doi: 10.1016/j.mce.2014.06.009. Epub 2014 Jun 19.

Glabridin induces glucose uptake via the AMP-activated protein kinase pathway in muscle cells.

Author information

1
Department of Agrobioscience, Graduate School of Agricultural Science, Nada-ku, Kobe University, Kobe 657-8501, Japan.
2
QOL Division, Kaneka Corporation, Kita-ku, Osaka 530-8288, Japan.
3
Department of Agrobioscience, Graduate School of Agricultural Science, Nada-ku, Kobe University, Kobe 657-8501, Japan. Electronic address: ashida@kobe-u.ac.jp.

Abstract

The present study demonstrates that glabridin, a prenylated isoflavone in licorice, stimulates glucose uptake through the adenosine monophosphate-activated protein kinase (AMPK) pathway in L6 myotubes. Treatment with glabridin for 4h induced glucose uptake in a dose-dependent manner accompanied by the translocation of glucose transporter type 4 (GLUT4) to the plasma membrane. Glabridin needed at least 4h to increase glucose uptake, while it significantly decreased glycogen and increased lactic acid within 15 min. Pharmacological inhibition of AMPK by Compound C suppressed the glabridin-induced glucose uptake, whereas phosphoinositide 3-kinase and Akt inhibition by LY294002 and Akt1/2 inhibitor, respectively, did not. Furthermore, glabridin induced AMPK phosphorylation, and siRNA for AMPK completely abolished glabridin-induced glucose uptake. We confirmed that glabridin-rich licorice extract prevent glucose intolerance accompanied by the AMPK-dependent GLUT4 translocation in the plasma membrane of mice skeletal muscle. These results indicate that glabridin may possess a therapeutic effect on metabolic disorders, such as diabetes and hyperglycemia, by modulating glucose metabolism through AMPK in skeletal muscle cells.

KEYWORDS:

Adenosine monophosphate-activated protein kinase; Glabridin; Glucose transporter type 4; Glucose uptake; Muscle cells

PMID:
24953974
DOI:
10.1016/j.mce.2014.06.009
[Indexed for MEDLINE]

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