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Nanoscale. 2014 Aug 7;6(15):8521-6. doi: 10.1039/c4nr01951b.

Label-free, in situ SERS monitoring of individual DNA hybridization in microfluidics.

Author information

1
Department of Electrical and Computer Engineering, USA. wshih@uh.edu.

Abstract

We present label-free, in situ monitoring of individual DNA hybridization in microfluidics. By immobilizing molecular sentinel probes on nanoporous gold disks, we demonstrate sensitivity approaching the single-molecule limit via surface-enhanced Raman scattering which provides robust signals without photobleaching for more than an hour. We further demonstrate that a target concentration as low as 20 pM can be detected within 10 min under diffusion-limited transport.

PMID:
24953169
DOI:
10.1039/c4nr01951b
[Indexed for MEDLINE]

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