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Diagn Microbiol Infect Dis. 2014 Aug;79(4):483-5. doi: 10.1016/j.diagmicrobio.2014.05.010. Epub 2014 May 20.

Characteristics of KPC-2-producing Klebsiella pneumoniae (ST258) clinical isolates from outbreaks in 2 Mexican medical centers.

Author information

1
Departamento de Diagnóstico Epidemiológico, Centro de Investigaciones Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, Mexico.
2
Hospital Regional 1(o) Octubre ISSSTE, México, DF, Mexico.
3
Laboratorio de Vigilancia Epidemiológica, ISSSTE, México, DF, Mexico.
4
Departamento de Vigilancia y Control Epidemiológico del ISSSTE, México, DF, Mexico.
5
Departamento de Diagnóstico Epidemiológico, Centro de Investigaciones Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, Mexico. Electronic address: jsilva@insp.mx.

Abstract

The KPC-producing Klebsiella pneumoniae sequence type 258 (ST258) is an important pathogen widely spread in nosocomial infections. In this study, we identified the KPC-2-producing K. pneumoniae clinical isolates of 2 unrelated outbreaks that corresponded to pandemic strain ST258. The isolates showed high resistance to cephalosporins, carbapenems, quinolones, and colistin. The KPC-2-producing K. pneumoniae isolates were compared to the previously studied KPC-3-producing K. pneumoniae isolates from an outbreak in Mexico; they showed an unrelated pulsed-field gel electrophoresis fingerprinting pattern and a different plasmid profile. The KPC-2 carbapenemase gene was identified in two 230- and 270-kb non-conjugative plasmids; however, 1 isolate transferred the KPC-2 gene onto an 80-kb plasmid. These findings endorse the need of carrying out a continuous molecular epidemiological surveillance of carbapenem-resistant isolates in hospitals in Mexico.

KEYWORDS:

Carbapenemase; Enterobacteria; Imipenem

[Indexed for MEDLINE]

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