Format

Send to

Choose Destination
Mol Syst Biol. 2014 Jun 21;10:732. doi: 10.15252/msb.20145172.

Cell cycle population effects in perturbation studies.

Author information

1
Molecular Cancer Research, University Medical Center Utrecht, Utrecht, the Netherlands.
2
Molecular Cancer Research, University Medical Center Utrecht, Utrecht, the Netherlands f.c.p.holstege@umcutrecht.nl.

Abstract

Growth condition perturbation or gene function disruption are commonly used strategies to study cellular systems. Although it is widely appreciated that such experiments may involve indirect effects, these frequently remain uncharacterized. Here, analysis of functionally unrelated Saccharyomyces cerevisiae deletion strains reveals a common gene expression signature. One property shared by these strains is slower growth, with increased presence of the signature in more slowly growing strains. The slow growth signature is highly similar to the environmental stress response (ESR), an expression response common to diverse environmental perturbations. Both environmental and genetic perturbations result in growth rate changes. These are accompanied by a change in the distribution of cells over different cell cycle phases. Rather than representing a direct expression response in single cells, both the slow growth signature and ESR mainly reflect a redistribution of cells over different cell cycle phases, primarily characterized by an increase in the G1 population. The findings have implications for any study of perturbation that is accompanied by growth rate changes. Strategies to counter these effects are presented and discussed.

KEYWORDS:

environmental stress response; gene deletion; gene expression; genome‐wide transcription; growth rate

PMID:
24952590
PMCID:
PMC4265054
DOI:
10.15252/msb.20145172
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center