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Bull Exp Biol Med. 2014 Jun;157(2):249-52. doi: 10.1007/s10517-014-2537-6. Epub 2014 Jun 22.

Biological effects of individually synthesized TNF-binding domain of variola virus CrmB protein.

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1
Research Institute of Clinical Immunology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia, tsdon@mail.ru.

Abstract

The biological characteristics of a 17-kDa protein synthesized in bacterial cells, a TNF-binding domain (VARV-TNF-BP) of a 47-kDa variola virus CrmB protein (VARV-CrmB) consisting of TNF-binding and chemokine-binding domains, were studied. Removal of the C-terminal chemokine-binding domain from VARV-CrmB protein was inessential for the efficiency of its inhibition of TNF cytotoxicity towards L929 mouse fibroblast culture and for TNF-induced oxidative metabolic activity of mouse blood leukocytes. The results of this study could form the basis for further studies of VARV-TNF-BP mechanisms of activity for prospective use in practical medicine.

PMID:
24952494
DOI:
10.1007/s10517-014-2537-6
[Indexed for MEDLINE]
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