Format

Send to

Choose Destination
Bioresour Technol. 2014 Aug;166:541-7. doi: 10.1016/j.biortech.2014.05.097. Epub 2014 Jun 3.

Physicochemical parameters optimization, and purification of phycobiliproteins from the isolated Nostoc sp.

Author information

1
Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, West Bengal, India.
2
Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, West Bengal, India. Electronic address: ddas.iitkgp@gmail.com.

Abstract

The present study investigated the effects of several physicochemical parameters on the improvement of phycobiliproteins (especially phycocyanin) synthesis in a newly isolated species of Nostoc sp. Standard BG11₀ medium was modified to enhance the biomass productivity in different photobioreactors. The initial pH of 8, light intensity of 40 μmol m(-2)s(-1), temperature of 35 °C, diurnal cycle of 16:8 h (light:dark regime), 75.48 μM Na₂CO₃ and 17.65 mM NaNO₃ were found most suitable for the phycobiliproteins synthesis. Cyanobacteria exhibited chromatic adaptation, causing overexpression of phycocyanin in red and phycoerythrin in green light. The maximum phycobiliproteins yield of 0.13 gg(-1) dry cell weight was obtained in green light. Phycocyanin was further purified using thin layer chromatography (TLC), anion exchange chromatography and SDS-PAGE (denaturing gel) electrophoresis.

KEYWORDS:

Cyanobacteria; Light; Phycocyanin; SDS–PAGE; pH

PMID:
24951941
DOI:
10.1016/j.biortech.2014.05.097
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center