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Anim Reprod Sci. 2014 Aug;148(3-4):221-7. doi: 10.1016/j.anireprosci.2014.05.012. Epub 2014 Jun 4.

Ovulation-inducing factor (OIF/NGF) from seminal plasma origin enhances Corpus Luteum function in llamas regardless the preovulatory follicle diameter.

Author information

1
Escuela de Medicina Veterinaria, Universidad Católica de Temuco, Temuco, Chile; Núcleo de investigación en Producción Alimentaria, Universidad Católica de Temuco, Temuco, Chile.
2
Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile; Programa de Doctorado en Ciencias Veterinarias, Escuela de Postgrado, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile.
3
Escuela de Medicina Veterinaria, Universidad Católica de Temuco, Temuco, Chile.
4
Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile.
5
Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
6
Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile; Ross University School of Veterinary Medicine, Basseterre, St. Kitts, West Indies. Electronic address: marceloratto@uach.cl.

Abstract

Ovulation-inducing factor (OIF) is a protein present in llama seminal plasma that has recently been identified as β-Nerve Growth Factor (NGF) and it induces not only a high rate of ovulation but also appears to have luteotrophic properties in this species. A 2-by-2 experimental design was used to determine the effect of treatments (OIF/NGF vs GnRH) and categories of preovulatory follicle diameter (7-10 vs >10mm) on ovulation rate, CL diameter and function in llamas. Llamas (n=32 llamas per group) were randomly assigned to receive an intramuscular dose of: (a) 1mg purified OIF/NGF in the presence of a follicle of 7-10mm in diameter; (b) 50 μg of GnRH in the presence of a follicle of 7-10mm in diameter; (c) 1mg purified OIF/NGF in the presence of a follicle >10mm in diameter; (d) 50 μg of GnRH in the presence of a follicle >10mm in diameter. Llamas were examined by ultrasonography every 12h from treatment to Day 2 (Day 0=treatment) to detect ovulation, and again on Day 8 to determine CL diameter. Ovulation rates did not differ among groups. There was an effect of preovulatory follicle size on Corpus Luteum diameter at Day 8 (P<0.001), however plasma progesterone concentration (n=15/per group) was higher (P<0.05) in the OIF/NGF - than that of the GnRH - treated group by the same day. We conclude that OIF/NGF treatment enhances CL function regardless preovulatory follicle size at the time of treatment.

KEYWORDS:

Corpus Luteum; Follicles; Llama; Nerve Growth Factor; Ovulation-inducing factor

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