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PLoS One. 2014 Jun 20;9(6):e100042. doi: 10.1371/journal.pone.0100042. eCollection 2014.

A microfluidic system for studying ageing and dynamic single-cell responses in budding yeast.

Author information

1
SynthSys - Center for Synthetic and Systems Biology and School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom.
2
Scottish Microelectronics Center, University of Edinburgh, Edinburgh, United Kingdom.

Abstract

Recognition of the importance of cell-to-cell variability in cellular decision-making and a growing interest in stochastic modeling of cellular processes has led to an increased demand for high density, reproducible, single-cell measurements in time-varying surroundings. We present ALCATRAS (A Long-term Culturing And TRApping System), a microfluidic device that can quantitatively monitor up to 1000 cells of budding yeast in a well-defined and controlled environment. Daughter cells are removed by fluid flow to avoid crowding allowing experiments to run for over 60 hours, and the extracellular media may be changed repeatedly and in seconds. We illustrate use of the device by measuring ageing through replicative life span curves, following the dynamics of the cell cycle, and examining history-dependent behaviour in the general stress response.

PMID:
24950344
PMCID:
PMC4065030
DOI:
10.1371/journal.pone.0100042
[Indexed for MEDLINE]
Free PMC Article

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