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J Ethnopharmacol. 2014 Aug 8;155(1):736-43. doi: 10.1016/j.jep.2014.06.026. Epub 2014 Jun 16.

The protective effect of Phoenix dactylifera L. seeds against CCl4-induced hepatotoxicity in rats.

Author information

1
Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, Helwan University, Cairo, Egypt. Electronic address: dalia_abdelaziz@pharm.helwan.edu.eg.
2
Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, Helwan University, Cairo, Egypt. Electronic address: ganah_nour@yahoo.com.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

In traditional Egyptian medicine, Phoenix dactylifera L. (date palm) seeds are listed in folk remedies for the management of diabetes, liver diseases and gastrointestinal disorders. The present study was conducted to investigate the protective effect of Phoenix dactylifera L. seeds aqueous suspension against the chemically-induced hepatic injury in rats.

METHODS:

Liver injury was achieved by exposing Wistar rats to CCl4 (10% in olive oil; 0.5 mL/rat; IP) twice a week for 4 weeks. Along with CCl4, aqueous suspensions of raw or roasted Phoenix dactylifera seeds (1.0 g/kg) were administered orally in a daily manner.

RESULTS:

Our results demonstrated that Phoenix dactylifera seeds significantly improved the CCl4-induced alterations in liver function parameters (AST, ALT, ALP and albumin). Moreover, the CCl4-induced oxidative stress, represented by elevated thiobarbituric acid reactive substance (TBARS), nitric oxide and oxidative DNA damage, was ameliorated by Phoenix dactylifera seeds treatment. In addition, Phoenix dactylifera seeds restored the activities of hepatic antioxidant enzymes (superoxide dismutase and glutathione S-transferase) that were declined after CCl4 treatment. Examination of liver histopathology revealed that Phoenix dactylifera seeds attenuate the incidence of liver lesions (including vacuolization and fibroblast proliferation) triggered by CCl4 intoxication.

CONCLUSION:

The Phoenix dactylifera seeds could be a promising candidate for protection against the CCl4-induced liver intoxication, and this hepatoprotective effect might be attributed to the antioxidant and free radical scavenging activities.

KEYWORDS:

Antioxidants; Carbon tetrachloride; Hepatoprotective; Oxidative stress; Phoenix dactylifera seeds

PMID:
24945397
DOI:
10.1016/j.jep.2014.06.026
[Indexed for MEDLINE]

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