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Plant Signal Behav. 2014 Jun 18;9. pii: e29579. [Epub ahead of print]

Time-sequential observation of spindle and phragmoplast orientation in BY-2 cells with altered cortical actin microfilament patterning.

Author information

1
Department of Integrated Biosciences; Graduate School of Frontier Sciences; The University of Tokyo; Kashiwa, Chiba, Japan.
2
Department of Life Science and Biotechnology; Faculty of Chemistry; Materials and Bioengineering; Kansai University; Yamate-cho, Suita, Osaka, Japan.

Abstract

Precise division plane determination is essential for plant development. At metaphase, a dense actin microfilament meshwork appears on both sides of the cell center, forming a characteristic cortical actin microfilament twin peak pattern in BY-2 cells. We previously reported a strong correlation between altered cortical actin microfilament patterning and an oblique mitotic spindle orientation, implying that these actin microfilament twin peaks play a role in the regulation of mitotic spindle orientation. In the present study, time-sequential observation was used to reveal the progression from oblique phragmoplast to oblique cell plate orientation in cells with altered cortical actin microfilament patterning. In contrast to cells with normal actin microfilament twin peaks, oblique phragmoplast reorientation was rarely observed in cells with altered cortical actin microfilament patterning. These results support the important roles of cortical actin microfilament patterning in division plane orientation.

KEYWORDS:

2,3,5-triiodobenzoic acid (TIBA); BY-YTRF1 cells; actin microfilament twin peaks; division plane orientation; jasplakinolide

PMID:
24941334

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