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Acta Microbiol Immunol Hung. 2014 Jun;61(2):107-19. doi: 10.1556/AMicr.61.2014.2.2.

Optimization of triacetylfusarinine C and ferricrocin productions in Aspergillus fumigatus.

Author information

1
University of Debrecen Department of Microbial Biotechnology and Cell Biology, Faculty of Science and Technology Debrecen Hungary.
2
University of Debrecen Department of Inorganic and Analytical Chemistry, Faculty of Science and Technology Debrecen Hungary.
3
Eszterházy Károly College Department of Zoology, Faculty of Sciences Eger Hungary.
4
University of Debrecen Department of Nephrology, Medical and Health Science Center Debrecen Hungary Hungarian Academy of Sciences Vascular Biology, Thrombosis and Hemostasis Research Group Debrecen Hungary.
5
Hungarian Academy of Sciences Vascular Biology, Thrombosis and Hemostasis Research Group Debrecen Hungary University of Debrecen Department of Neonatology, Medical and Health Science Center Debrecen Hungary.
6
University of Debrecen Department of Physiology, Medical and Health Science Center Debrecen Hungary.

Abstract

Iron is an essential element for all microorganisms. Bacteria and fungi produce versatile siderophores for binding and storing this essential transition metal when its availability is limited in the environment. The aim of the study was to optimize the fermentation medium of Aspergillus fumigatus for siderophore production. Triacetyl-fusarinine C and ferricrocin yields were dependent on glucose and glycine supplementations as well as the initial pH of the culture media. The optimal fermentation medium for triacetylfusarinine C production contained 8% glucose, 0.4% glycine and the initial pH was set to 5.9. Meanwhile, maximal ferricrocin yields were recorded in the presence of 10% glucose, 0.5% glycine and at an initial pH of 7.4. Under optimized fermentation conditions, the yields for triacetylfusarinine C and ferricrocin increased up to 2.9 g/l culture medium and 18.9 mg/g mycelium, respectively.

KEYWORDS:

Aspergillus fumigatus Af293; ferricrocin; response surface methodology; triacetylfusarinine C

PMID:
24939680
DOI:
10.1556/AMicr.61.2014.2.2
[Indexed for MEDLINE]

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