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Biochem Biophys Res Commun. 2014 Jul 18;450(1):318-23. doi: 10.1016/j.bbrc.2014.05.113. Epub 2014 Jun 2.

Ablation of Elovl6 protects pancreatic islets from high-fat diet-induced impairment of insulin secretion.

Author information

1
Department of Internal Medicine (Endocrinology and Metabolism), Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan.
2
Department of Internal Medicine (Endocrinology and Metabolism), Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan. Electronic address: t-matsuz@md.tsukuba.ac.jp.
3
Experimental Animal Laboratory, Research Center for Genomic Medicine, Saitama Medical University, 1397-1 Yamane, Hidaka City, Saitama 350-1241, Japan.
4
Department of Internal Medicine, Faculty of Medicine, Niigata University, 1-754 Asahimachi, Niigata, Niigata 951-8510, Japan.

Abstract

ELOVL family member 6, elongation of very long-chain fatty acids (Elovl6) is a microsomal enzyme that regulates the elongation of C12-16 saturated and monounsaturated fatty acids and is related to the development of obesity-induced insulin resistance via the modification of the fatty acid composition. In this study, we investigated the role of systemic Elovl6 in the pancreatic islet and β-cell function. Elovl6 is expressed in both islets and β-cell lines. In mice fed with chow, islets of the Elovl6(-/-) mice displayed normal architecture and β-cell mass compared with those of the wild-type mice. However, when fed a high-fat, high-sucrose (HFHS) diet, the islet hypertrophy in response to insulin resistance observed in normal mice was attenuated and glucose-stimulated insulin secretion (GSIS) increased in the islets of Elovl6(-/-) mice compared with those of the wild-type mice. Enhanced GSIS in the HFHS Elovl6(-/-) islets was associated with an increased ATP/ADP ratio and the suppression of ATF-3 expression. Our findings suggest that Elovl6 could be involved in insulin secretory capacity per β-cell and diabetes.

KEYWORDS:

Fatty acid; Insulin; Islet; Obesity; Type 2 diabetes; β-cell

PMID:
24938128
DOI:
10.1016/j.bbrc.2014.05.113
[Indexed for MEDLINE]

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