Format

Send to

Choose Destination
FEBS Lett. 2014 Aug 25;588(17):2814-21. doi: 10.1016/j.febslet.2014.06.027. Epub 2014 Jun 14.

Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast.

Author information

1
Laboratory of Cell Regulation, Cancer Research UK, London Research Institute, Lincoln's Inn Fields Laboratories, 44 Lincoln's Inn Fields, London WC2A 3LY, UK.
2
Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Tokyo 113-0033, Japan.
3
Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Tokyo 113-0033, Japan; Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University, Center for Advanced Biomedical Sciences (TWIns), 2-2 Wakamatsucho, Shinjuku, Tokyo 162-8480, Japan.
4
Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Tokyo 113-0033, Japan; Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University, Center for Advanced Biomedical Sciences (TWIns), 2-2 Wakamatsucho, Shinjuku, Tokyo 162-8480, Japan; PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
5
Laboratory of Cell Regulation, Cancer Research UK, London Research Institute, Lincoln's Inn Fields Laboratories, 44 Lincoln's Inn Fields, London WC2A 3LY, UK. Electronic address: takashi.toda@cancer.org.uk.

Abstract

The conserved TACC protein family localises to the centrosome (the spindle pole body, SPB in fungi) and mitotic spindles, thereby playing a crucial role in bipolar spindle assembly. However, it remains elusive how TACC proteins are recruited to the centrosome/SPB. Here, using fission yeast Alp7/TACC, we have determined clustered five amino acid residues within the TACC domain required for SPB localisation. Critically, these sequences are essential for the functions of Alp7, including proper spindle formation and mitotic progression. Moreover, we have identified pericentrin-like Pcp1 as a loading factor to the mitotic SPB, although Pcp1 is not a sole platform.

KEYWORDS:

Centrosome; Fission yeast; Pericentrin family; Spindle microtubule; Spindle pole body; TACC

PMID:
24937146
PMCID:
PMC4158419
DOI:
10.1016/j.febslet.2014.06.027
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center