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Stem Cell Reports. 2014 Apr 24;2(5):662-74. doi: 10.1016/j.stemcr.2014.03.011. eCollection 2014.

Transplantation of embryonic and induced pluripotent stem cell-derived 3D retinal sheets into retinal degenerative mice.

Author information

1
Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan ; Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.
2
Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan.
3
Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan ; Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
4
Organogenesis and Neurogenesis Group, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan.
5
Electron Microscope Laboratory, RIKEN Center for Developmental Biology, Kobe 650-0047, Japan.

Abstract

In this article, we show that mouse embryonic stem cell- or induced pluripotent stem cell-derived 3D retinal tissue developed a structured outer nuclear layer (ONL) with complete inner and outer segments even in an advanced retinal degeneration model (rd1) that lacked ONL. We also observed host-graft synaptic connections by immunohistochemistry. This study provides a "proof of concept" for retinal sheet transplantation therapy for advanced retinal degenerative diseases.

PMID:
24936453
PMCID:
PMC4050483
DOI:
10.1016/j.stemcr.2014.03.011
[Indexed for MEDLINE]
Free PMC Article
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