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Int Immunopharmacol. 2014 Aug;21(2):474-80. doi: 10.1016/j.intimp.2014.06.002. Epub 2014 Jun 13.

HJB-1, a 17-hydroxy-jolkinolide B derivative, inhibits LPS-induced inflammation in mouse peritoneal macrophages.

Author information

  • 1Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, PR China.
  • 2Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, PR China; School of Life Sciences and Technology, Tongji University, Shanghai 200092, PR China.
  • 3Central Laboratory, The Second Clinical Hospital, Jilin University, Changchun 130041, PR China.
  • 4Jilin Provincial Center for Disease Control and Prevention, 130062, PR China.
  • 5Dept. of Biomedical Sciences, Ross University School of Veterinary Medicine, Saint Kitts and Nevis.
  • 6School of Life Sciences and Technology, Tongji University, Shanghai 200092, PR China. Electronic address: hbwang@tongji.edu.cn.
  • 7Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, PR China. Electronic address: wanchunsun@jlu.edu.cn.

Abstract

Jolkinolide B (JB) and 17-hydroxy-JB (HJB) are diterpenoids from plants and it has been reported that the presence of a C-17 hydroxy group in JB significantly enhances the anti-inflammatory potency of JB. In this study, two HJB derivatives HJB-1 and HJB-2 were generated by the chemical modification of a 17-hydroxy group of HJB. HJB-1 more effectively inhibited TNF-α, IL-1β and IL-6 release in LPS-stimulated mouse peritoneal macrophages. In addition, HJB-1 reduced LPS-induced mRNA expression of TNF-α, IL-1β, IL-6, COX-2 and iNOS in a concentration-dependent manner, but did not alter IL-10 mRNA expression. LPS-induced NF-κB activation and MAPK phosphorylation were also effectively inhibited by HJB-1. These results demonstrate that HJB-1 exerts anti-inflammatory effects on LPS-activated mouse peritoneal macrophages by inhibiting NF-κB activation and MAPK phosphorylation and modification of a 17-hydroxy group of HJB may enhance the anti-inflammatory potency of HJB derivatives.

KEYWORDS:

17-Hydroxy-jolkinolide B; Inflammation; LPS; MAPK; NF-κB

PMID:
24933588
DOI:
10.1016/j.intimp.2014.06.002
[PubMed - indexed for MEDLINE]
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