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ACS Synth Biol. 2014 Oct 17;3(10):708-16. doi: 10.1021/sb400137b. Epub 2014 Feb 13.

Transcription activator-like effectors: a toolkit for synthetic biology.

Author information

1
Bioengineering Department, The University of Texas at Dallas , 800 West Campbell Road, Richardson, Texas 75080 United States.

Abstract

Transcription activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria to aid the infection of plant species. TALEs assist infections by binding to specific DNA sequences and activating the expression of host genes. Recent results show that TALE proteins consist of a central repeat domain, which determines the DNA targeting specificity and can be rapidly synthesized de novo. Considering the highly modular nature of TALEs, their versatility, and the ease of constructing these proteins, this technology can have important implications for synthetic biology applications. Here, we review developments in the area with a particular focus on modifications for custom and controllable gene regulation.

PMID:
24933470
PMCID:
PMC4210167
DOI:
10.1021/sb400137b
[Indexed for MEDLINE]
Free PMC Article

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