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Angew Chem Int Ed Engl. 2014 Jul 28;53(31):8137-41. doi: 10.1002/anie.201404043. Epub 2014 Jun 16.

Single-molecule mechanochemical sensing using DNA origami nanostructures.

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Department of Chemistry & Biochemistry and School of Biomedical Sciences, Kent State University, Kent, OH 44240 (USA).


While single-molecule sensing offers the ultimate detection limit, its throughput is often restricted as sensing events are carried out one at a time in most cases. 2D and 3D DNA origami nanostructures are used as expanded single-molecule platforms in a new mechanochemical sensing strategy. As a proof of concept, six sensing probes are incorporated in a 7-tile DNA origami nanoassembly, wherein binding of a target molecule to any of these probes leads to mechanochemical rearrangement of the origami nanostructure, which is monitored in real time by optical tweezers. Using these platforms, 10 pM platelet-derived growth factor (PDGF) are detected within 10 minutes, while demonstrating multiplex sensing of the PDGF and a target DNA in the same solution. By tapping into the rapid development of versatile DNA origami nanostructures, this mechanochemical platform is anticipated to offer a long sought solution for single-molecule sensing with improved throughput.


DNA nanotechnology; DNA origami nanostructures; mechanochemical sensing; optical tweezers; single-molecule techniques

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