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J Diabetes Complications. 2014 Sep-Oct;28(5):689-97. doi: 10.1016/j.jdiacomp.2014.05.001. Epub 2014 May 15.

Enhanced proliferation in colorectal epithelium of patients with type 2 diabetes correlates with β-catenin accumulation.

Author information

1
Department of Gastroenterology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 107 Yan Jiang Xi Road, Guangzhou, Guangdong 510120, People's Republic of China.
2
Department of Gastroenterology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 107 Yan Jiang Xi Road, Guangzhou, Guangdong 510120, People's Republic of China. Electronic address: qikuichen@yahoo.com.

Abstract

AIMS:

β-Catenin accumulation promotes proliferation. However, the correlation between proliferation of colorectal epithelium and β-catenin in type 2 diabetes mellitus (DM) patients remains unclear.

METHODS:

Colorectal epithelium samples from distal ends of colorectal adenocarcinomas without histological aberrances were divided into two groups: DM patients with type 2 DM for more than 1year (n=27) and non-DM patients without hyperglycemia (n=20). Samples from patients without colorectal epithelial disease or hyperglycemia served as a control group (n=6). Proliferative index was calculated as the percentage of proliferating cell nuclear antigen positive cells. Wnt/β-catenin signaling was assessed immunohistochemically and phosphorylation of β-catenin was assessed by immunofluorescence.

RESULTS:

Compared with the non-DM or control group, the proliferative index and expression of lactate dehydrogenase A and Wnt/β-catenin signaling were significantly higher in the DM group (all p<0.01). The proliferative index correlated positively with β-catenin expression (Spearman correlation coefficient=0.55; p<0.01). Reduced phosphorylation at serine 33/37 and increased phosphorylation at serine 675 of β-catenin were detected in the DM group (all p<0.01).

CONCLUSIONS:

Enhanced proliferation, accompanied by increased aerobic glycolysis, was detected in colorectal epithelium of patients with diabetes. β-Catenin accumulation with altered phosphorylation correlated with the proliferative changes.

KEYWORDS:

Colorectal epithelium; Diabetes; Phosphorylation; Proliferation; β-Catenin

PMID:
24930713
DOI:
10.1016/j.jdiacomp.2014.05.001
[Indexed for MEDLINE]
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