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Food Microbiol. 2014 Sep;42:166-71. doi: 10.1016/j.fm.2014.03.021. Epub 2014 Apr 2.

Multilocus sequence typing analysis and antibiotic resistance of Clostridium difficile strains isolated from retail meat and humans in Belgium.

Author information

1
Food Science Department, Faculty of Veterinary Medicine, University of Liège, B43bis, Sart-Tilman, 4000 Liège, Belgium. Electronic address: kriszsi@hotmail.com.
2
Food Science Department, Faculty of Veterinary Medicine, University of Liège, B43bis, Sart-Tilman, 4000 Liège, Belgium.
3
Microbiology Unit, Catholic University of Louvain, Avenue Hippocrate 5490, 1200 Brussels, Belgium.

Abstract

Clostridium difficile has been isolated from food animals and meat, specially ground pork and ground beef. The recovered isolates were closely related to C. difficile human strains, indicating that animals and food are possible transmission routes of human C. difficile infection. The main objective of this study was to characterize C. difficile isolates from retail meat and to compare with human isolates recovered from hospital patients in Belgium. Raw meat (beef and pork) was obtained from the retail trade. C. difficile was recovered from 2.3% of the beef samples and from 4.7% of the pork samples. A total of 4 different PCR-ribotypes were identified with a large percentage of types 078 and 014. Resistance to moxifloxacin and erythromycin was detected. The multi-locus sequence typing (MLST) analysis showed that meat and human isolates cluster in the same lineage. This study reveals the presence of toxigenic C. difficile in retail meat in Belgium with predominance PCR-ribotypes 078 and 014, which are among the four most prevalent ribotypes of C. difficile isolated from humans in Europe.

KEYWORDS:

Clostridium difficile; Multilocus sequence typing analysis; Retail meat

PMID:
24929733
DOI:
10.1016/j.fm.2014.03.021
[Indexed for MEDLINE]

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