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Nat Cell Biol. 2014 Jul;16(7):639-51. doi: 10.1038/ncb2985. Epub 2014 Jun 15.

Adherens junction treadmilling during collective migration.

Author information

1
1] Institut Pasteur - CNRS URA 2582, Cell Polarity, Migration and Cancer Unit, 25 rue du Dr Roux 75724 Paris Cedex 15, France [2] Université Pierre et Marie Curie, Cellule Pasteur UPMC, rue du Dr Roux Paris 75015, France.
2
Institut Pasteur - CNRS URA 2582, Cell Polarity, Migration and Cancer Unit, 25 rue du Dr Roux 75724 Paris Cedex 15, France.

Abstract

Collective cell migration is essential for both physiological and pathological processes. Adherens junctions (AJs) maintain the integrity of the migrating cell group and promote cell coordination while allowing cellular rearrangements. Here, we show that AJs undergo a continuous treadmilling along the lateral sides of adjacent leading cells. The treadmilling is driven by an actin-dependent rearward movement of AJs and is supported by the polarized recycling of N-cadherin. N-cadherin is mainly internalized at the cell rear and then recycled to the leading edge where it accumulates before being incorporated into forming AJs at the front of lateral cell-cell contacts. The polarized dynamics of AJs is controlled by a front-to-rear gradient of p120-catenin phosphorylation, which regulates polarized trafficking of N-cadherin. Perturbation of the GSK3-dependent phosphorylation of p120-catenin impacts on the stability of AJs, and the polarity and speed of leading cells during collective migration.

PMID:
24929360
DOI:
10.1038/ncb2985
[Indexed for MEDLINE]

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