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J Immunol. 2014 Jul 15;193(2):529-39. doi: 10.4049/jimmunol.1303247. Epub 2014 Jun 13.

B cells regulate CD4+ T cell responses to papain following B cell receptor-independent papain uptake.

Author information

1
Division of Rheumatology, Immunology, and Allergy, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115; and.
2
Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA 02115.
3
Division of Rheumatology, Immunology, and Allergy, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115; and mgurish@partners.org.

Abstract

Papain, a cysteine protease allergen with inherent adjuvant activity, induces potent IL-4 expression by T cells in the popliteal lymph nodes of mice following footpad immunization. In this study, we identify a novel, non-BCR-mediated capacity for B cells to rapidly bind and internalize papain. B cells subsequently regulate the adaptive immune response by enhancing ICOS expression on CD4(+) T cells and amplifying Th2 and follicular helper T cell induction. Ab blockade of ICOS ligand, expressed by popliteal lymph node B cells, but not dendritic cells, at the peak of the response inhibits IL-4 responses in wild-type mice but not B cell-deficient mice. Thus, B cells play a critical role in amplifying adjuvant-dependent Th2 polarization following noncanonical acquisition and internalization of the cysteine protease papain.

PMID:
24928991
PMCID:
PMC4203309
DOI:
10.4049/jimmunol.1303247
[Indexed for MEDLINE]
Free PMC Article

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