Format

Send to

Choose Destination
Proc Natl Acad Sci U S A. 2014 Jun 24;111(25):9271-6. doi: 10.1073/pnas.1406110111. Epub 2014 Jun 9.

Marker for type VI secretion system effectors.

Author information

1
Department of Molecular Biology.
2
Howard Hughes Medical Institute.
3
Department of Biochemistry, and.
4
Howard Hughes Medical Institute,Department of Biochemistry, andDepartment of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX 75390-8816.
5
Department of Molecular Biology,Department of Biochemistry, and kim.orth@utsouthwestern.edu.

Abstract

Bacteria use diverse mechanisms to kill, manipulate, and compete with other cells. The recently discovered type VI secretion system (T6SS) is widespread in bacterial pathogens and used to deliver virulence effector proteins into target cells. Using comparative proteomics, we identified two previously unidentified T6SS effectors that contained a conserved motif. Bioinformatic analyses revealed that this N-terminal motif, named MIX (marker for type six effectors), is found in numerous polymorphic bacterial proteins that are primarily located in the T6SS genome neighborhood. We demonstrate that several MIX-containing proteins are T6SS effectors and that they are not required for T6SS activity. Thus, we propose that MIX-containing proteins are T6SS effectors. Our findings allow for the identification of numerous uncharacterized T6SS effectors that will undoubtedly lead to the discovery of new biological mechanisms.

KEYWORDS:

bacterial competition; colicin; toxin; vibrio

PMID:
24927539
PMCID:
PMC4078801
DOI:
10.1073/pnas.1406110111
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center