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Proc Natl Acad Sci U S A. 2014 Jun 24;111(25):9229-34. doi: 10.1073/pnas.1318731111. Epub 2014 Jun 10.

Human memory T cells from the bone marrow are resting and maintain long-lasting systemic memory.

Author information

1
Cell Biology.
2
Bioinformatics.
3
Cell Biology,Signal Transduction, and.
4
Core Unit Cell Harvesting, Berlin-Brandenburg Center for Regenerative Therapies and.
5
Center for Musculoskeletal Surgery and.
6
Department of Gastroenterology, Infectiology and Rheumatology, Charité University of Medicine Berlin, 12200 Berlin, Germany.
7
Clinical Tumor Immunology and Immunemonitoring, Institute for Medical Immunology, Charité University of Medicine Berlin, 13353 Berlin, Germany;
8
Osteoimmunology, Deutsches Rheuma-Forschungszentrum Berlin, 10117 Berlin, Germany;
9
Institute of Virology, Charité University of Medicine Berlin, 10117 Berlin, Germany; and.
10
Cell Biology, dong@drfz.de.

Abstract

In the bone marrow, a population of memory T cells has been described that promotes efficient secondary immune responses and has been considered to be preactivated, owing to its expression of CD69 and CD25. Here we show that human bone marrow professional memory T cells are not activated but are resting in terms of proliferation, transcription, and mobility. They are in the G0 phase of the cell cycle, and their transcriptome is that of resting T cells. The repertoire of CD4(+) bone marrow memory T cells compared with CD4(+) memory T cells from the blood is significantly enriched for T cells specific for cytomegalovirus-pp65 (immunodominant protein), tetanus toxoid, measles, mumps, and rubella. It is not enriched for vaccinia virus and Candida albicans-MP65 (immunodominant protein), typical pathogens of skin and/or mucosa. CD4(+) memory T cells specific for measles are maintained nearly exclusively in the bone marrow. Thus, CD4(+) memory T cells from the bone marrow provide long-term memory for systemic pathogens.

KEYWORDS:

antigen-specific response; polyfunctional; short- and long-term memory

PMID:
24927527
PMCID:
PMC4078840
DOI:
10.1073/pnas.1318731111
[Indexed for MEDLINE]
Free PMC Article

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