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Anticancer Res. 2014 Jun;34(6):3211-5.

Microsatellite instability analysis in uterine cavity washings to detect endometrial cancer in Lynch syndrome.

Author information

1
Université Paris Descartes, Sorbonne Paris Cité, Faculté de Médecine, Paris, France Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges-Pompidou, Chirurgie Cancérologique Gynécologique et du Sein, Paris, France INSERM UMR-S 747, Université Paris Descartes, Sorbonne Paris Cité, Paris, France anne-sophie.bats@egp.aphp.fr.
2
Université Paris Descartes, Sorbonne Paris Cité, Faculté de Médecine, Paris, France Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges-Pompidou, Biochimie, Paris, France INSERM UMR S775, Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
3
Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges-Pompidou, Biochimie, Paris, France.
4
INSERM UMR-S 747, Université Paris Descartes, Sorbonne Paris Cité, Paris, France Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges-Pompidou, Anatomo-pathologie, Paris, France.
5
Université Paris Descartes, Sorbonne Paris Cité, Faculté de Médecine, Paris, France Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges-Pompidou, Chirurgie Cancérologique Gynécologique et du Sein, Paris, France INSERM UMR-S 747, Université Paris Descartes, Sorbonne Paris Cité, Paris, France.

Abstract

AIM:

To assess the feasibility of Microsatellite Instability (MSI) analysis in uterine cavity washings for detecting endometrial cancer in Lynch syndrome.

MATERIALS AND METHODS:

This was a proof-of-concept study in Lynch syndrome patients, scheduled for hysterectomy. At the beginning of surgical procedure, uterine cavity washings were performed, and sent for MSI analysis. Pathological examination of the uterus was associated with mismatch repair protein expression and MSI analysis.

RESULTS:

Nine patients were included in the study. Uterine cavity washings were feasible and interpretable in all cases. Final histological report identified 2 endometrial cancers and 7 benign specimens. There was no atypical hyperplasia. Sensitivity, specificity, positive predictive value, and negative predictive value of MSI analysis in uterine washings reached 100% in all cases. Concordance of MSI presence or absence was absolute between uterine washings and final histology.

CONCLUSION:

MSI analysis in uterine cavity washings may be a promising screening tool for Lynch syndrome-associated endometrial cancer diagnosis.

KEYWORDS:

Endometrial cancer; Lynch syndrome; microsatellite instability analysis; screening; uterine cavity washings

PMID:
24922696
[Indexed for MEDLINE]

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