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Pharm Biol. 2014 Jul;52(7):841-7. doi: 10.3109/13880209.2013.871639. Epub 2014 Feb 7.

Screening compounds of Chinese medicinal herbs anti-Marek's disease virus.

Author information

1
College of Animal Science and Veterinary Medicine, Shanxi Agricultural University , Taigu, Shanxi , PR China .

Abstract

CONTEXT:

Marek's disease (MD) seriously threatens the world poultry industry and has resulted in great economic losses. Chinese medicinal herbs are a rich source for lead compounds and drug candidates for antiviral treatments.

OBJECTIVE:

To investigate the anti-MDV activity and mechanism of 20 compounds extracted from Chinese medicinal herbs.

MATERIALS AND METHODS:

Antiviral assay, time of addition experiments, and virucidal assay were performed on chicken embryo fibroblast cells. The 50% cytotoxic concentration and 50% effective concentration were determined and, accordingly, selectivity index and inhibition ratio were calculated.

RESULTS:

Antiviral assay showed dipotassium glycyrrhizinate (DG) and sodium tanshinone IIA sulfonate (STS) exhibited significantly inhibitory activity against MDV in a dose-dependent manner. EC50 of DG and STS were 893.5 ± 36.99 µg/mL and 54.82 ± 2.99 µg/mL, and selective index (SI) were >3.36 and >9.12, respectively. Time of addition experiment and virucidal assay demonstrated DG inhibited viral replication in the full replication cycle and inactivated MDV particles in non-time-dependent manner, but STS interfered with the early stage of MDV replication and inactivated MDV particles in a time-dependent manner. Moreover, both DG and STS promoted apoptosis of cells infected by MDV.

DISCUSSION AND CONCLUSION:

DG and STS have great potential for developing new anti-MDV drugs for clinic application.

KEYWORDS:

Antiviral; MDV; apoptosis; dipotassium glycyrrhizinate; sodium tanshinone IIA sulfonate

PMID:
24920229
DOI:
10.3109/13880209.2013.871639
[Indexed for MEDLINE]

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