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J Microbiol Methods. 2014 Sep;104:19-25. doi: 10.1016/j.mimet.2014.05.024. Epub 2014 Jun 8.

Analysis of species and intra-species associations between the Mycobacterium abscessus complex strains using pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST).

Author information

1
Division of Tuberculosis and Bacterial Respiratory Infections, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Chungbuk, Republic of Korea.
2
Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Republic of Korea.
3
Department of Microbiology and Immunology, Biomedical Sciences, Liver Research Institute, Cancer Research Institute and Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul, Republic of Korea. Electronic address: kbumjoon@snu.ac.kr.
4
Division of Tuberculosis and Bacterial Respiratory Infections, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Chungbuk, Republic of Korea. Electronic address: kkingsh@chol.com.

Abstract

PFGE and MLST showed that the strains of M. massiliense hsp65 II-1 were clearly separated from the strains of M. massiliense hsp65 I or II-2 as well as the strains of M. abscessus or M. bolletii; thus, M. massiliense hsp6 5II-1 might represent an additional subspecies of M. massiliense.

KEYWORDS:

M. abscessus complex; M. bolletii; M. massiliense; MLST; PFGE

PMID:
24918987
DOI:
10.1016/j.mimet.2014.05.024
[Indexed for MEDLINE]

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