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Phytomedicine. 2014 Sep 15;21(10):1189-95. doi: 10.1016/j.phymed.2014.05.004. Epub 2014 Jun 7.

Antioxidative effects of Panax notoginseng saponins in brain cells.

Author information

1
Department of Neurosurgery, Medical School, University of Michigan, Ann Arbor, MI, USA; Department of Pharmacology, Yunnan University of Traditional Chinese Medicine, Kunming, China.
2
Department of Neurosurgery, Medical School, University of Michigan, Ann Arbor, MI, USA.
3
Department of Neurosurgery, Medical School, University of Michigan, Ann Arbor, MI, USA; Department of Molecular and Integrative Physiology, Medical School, University of Michigan, Ann Arbor, MI, USA.
4
Department of Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming, China.
5
Department of Neurosurgery, Medical School, University of Michigan, Ann Arbor, MI, USA. Electronic address: jmxxdrnx@umich.edu.

Abstract

Oxidative stress resulting from accumulation of reactive oxygen species (ROS) is involved in cell death associated with neurological disorders such as stroke, Alzheimer's disease and traumatic brain injury. Antioxidant compounds that improve endogenous antioxidant defenses have been proposed for neural protection. The purpose of this study was to investigate the potential protective effects of total saponin in leaves of Panax notoginseng (LPNS) on oxidative stress and cell death in brain cells in vitro. Lactate dehydrogenase (LDH) assay indicated that LPNS (5 μg/ml) reduced H2O2-induced cell death in primary rat cortical astrocytes (23±8% reduction in LDH release vs. control). Similar protection was found in oxygen and glucose deprivation/reoxygenation induced SH-SY5Y (a human neuroblastoma cell line) cell damage (78±7% reduction vs. control). The protective effects of LPNS in astrocytes were associated with attenuation of reactive oxygen species (ROS) accumulation. These effects involved activation of Nrf2 (nuclear translocation) and upregulation of downstream antioxidant systems including heme oxygenase-1 (HO-1) and glutathione S-transferase pi 1 (GSTP1). These results demonstrate for the first time that LPNS has antioxidative effects which may be neuroprotective in neurological disorders.

KEYWORDS:

Astrocytes; Oxidative stress; SH-SY5Y cells; Total saponins of Panax notoginseng leaves

PMID:
24916704
PMCID:
PMC4111966
DOI:
10.1016/j.phymed.2014.05.004
[Indexed for MEDLINE]
Free PMC Article

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