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Mol Biol Evol. 2014 Sep;31(9):2356-64. doi: 10.1093/molbev/msu185. Epub 2014 Jun 10.

Evolutionary origins of human herpes simplex viruses 1 and 2.

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Department of Medicine, University of California, San Diego
Bioinformatics and Systems Biology Graduate Program, University of California, San Diego.
Department of Medicine, University of California, San DiegoVeterans Affairs San Diego Healthcare System, San Diego, CA.
Department of Medicine, University of California, San DiegoDepartment of Mathematical Sciences, Stellenbosch University, Stellenbosch, South Africa.
Department of Medicine, University of California, San Diego.


Herpesviruses have been infecting and codiverging with their vertebrate hosts for hundreds of millions of years. The primate simplex viruses exemplify this pattern of virus-host codivergence, at a minimum, as far back as the most recent common ancestor of New World monkeys, Old World monkeys, and apes. Humans are the only primate species known to be infected with two distinct herpes simplex viruses: HSV-1 and HSV-2. Human herpes simplex viruses are ubiquitous, with over two-thirds of the human population infected by at least one virus. Here, we investigated whether the additional human simplex virus is the result of ancient viral lineage duplication or cross-species transmission. We found that standard phylogenetic models of nucleotide substitution are inadequate for distinguishing among these competing hypotheses; the extent of synonymous substitutions causes a substantial underestimation of the lengths of some of the branches in the phylogeny, consistent with observations in other viruses (e.g., avian influenza, Ebola, and coronaviruses). To more accurately estimate ancient viral divergence times, we applied a branch-site random effects likelihood model of molecular evolution that allows the strength of natural selection to vary across both the viral phylogeny and the gene alignment. This selection-informed model favored a scenario in which HSV-1 is the result of ancient codivergence and HSV-2 arose from a cross-species transmission event from the ancestor of modern chimpanzees to an extinct Homo precursor of modern humans, around 1.6 Ma. These results provide a new framework for understanding human herpes simplex virus evolution and demonstrate the importance of using selection-informed models of sequence evolution when investigating viral origin hypotheses.


co-divergence; cross-species transmission; homo; molecular clock; selection; zoonosis

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