Format

Send to

Choose Destination
See comment in PubMed Commons below
Acta Crystallogr F Struct Biol Commun. 2014 Jun;70(Pt 6):827-31. doi: 10.1107/S2053230X1400987X. Epub 2014 May 25.

Protein expression, characterization, crystallization and preliminary X-ray crystallographic analysis of a Fic protein from Clostridium difficile.

Author information

1
Microbiology and Infection Control, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen, Denmark.
2
Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands.
3
Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen, Denmark.
4
Department of Clinical Biochemistry and Immunology, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen, Denmark.

Abstract

Fic domains in proteins are found in abundance in nature from the simplest prokaryotes to animals. Interestingly, Fic domains found in two virulence factors of Gram-negative bacteria have recently been demonstrated to catalyse the transfer of the AMP moiety from ATP to small host GTPases. This post-translational modification has attracted considerable interest and a role for adenylylation in pathology and physiology is emerging. This work was aimed at the structural characterization of a newly identified Fic protein of the Gram-positive bacterium Clostridium difficile. A constitutively active inhibitory helix mutant of C. difficile Fic was overexpressed in Escherichia coli, purified and crystallized by the vapour-diffusion technique. Preliminary X-ray crystallographic analysis shows that the crystals diffract to at least 1.68 Å resolution at a synchrotron X-ray source. The crystals belonged to the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a=45.6, b=80.8, c=144.7 Å, α=β=γ=90°. Two molecules per asymmetric unit corresponds to a Matthews coefficient of 2.37 Å3 Da(-1) and a solvent content of 48%.

KEYWORDS:

Clostridium difficile; Fic protein; adenylylation

PMID:
24915103
PMCID:
PMC4051547
DOI:
10.1107/S2053230X1400987X
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for International Union of Crystallography Icon for PubMed Central
    Loading ...
    Support Center