Format

Send to

Choose Destination
See comment in PubMed Commons below
Genetics. 2014 Sep;198(1):219-28. doi: 10.1534/genetics.114.165290. Epub 2014 Jun 9.

Corolla is a novel protein that contributes to the architecture of the synaptonemal complex of Drosophila.

Author information

1
Stowers Institute for Medical Research, Kansas City, Missouri 64110.
2
Stowers Institute for Medical Research, Kansas City, Missouri 64110 Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas 66160.
3
Department of Ecology and Evolutionary Biology, University of Kansas, Lawrence, Kansas 66045.
4
Stowers Institute for Medical Research, Kansas City, Missouri 64110 Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas 66160 rsh@stowers.org.

Abstract

In most organisms the synaptonemal complex (SC) connects paired homologs along their entire length during much of meiotic prophase. To better understand the structure of the SC, we aim to identify its components and to determine how each of these components contributes to SC function. Here, we report the identification of a novel SC component in Drosophila melanogaster female oocytes, which we have named Corolla. Using structured illumination microscopy, we demonstrate that Corolla is a component of the central region of the SC. Consistent with its localization, we show by yeast two-hybrid analysis that Corolla strongly interacts with Cona, a central element protein, demonstrating the first direct interaction between two inner-synaptonemal complex proteins in Drosophila. These observations help provide a more complete model of SC structure and function in Drosophila females.

KEYWORDS:

chromosome segregation; meiosis; next-generation sequencing; structured illumination microscopy; synaptonemal complex

PMID:
24913682
PMCID:
PMC4174934
DOI:
10.1534/genetics.114.165290
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Support Center