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J Plant Physiol. 2014 Jul 1;171(11):907-14. doi: 10.1016/j.jplph.2014.02.011. Epub 2014 Mar 22.

The dihydrolipoyl acyltransferase gene BCE2 participates in basal resistance against Phytophthora infestans in potato and Nicotiana benthamiana.

Author information

1
National Center for Vegetable Improvement (Central China), Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
2
National Center for Vegetable Improvement (Central China), Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Xinjiang Production and Construction Corps Key Laboratory of Protection and Utilization of Biological Resources, Tarim Basin 843300, People's Republic of China.
3
National Center for Vegetable Improvement (Central China), Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: tianzhd@mail.hzau.edu.cn.
4
National Center for Vegetable Improvement (Central China), Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: xiech@mail.hzau.edu.cn.

Abstract

Dihydrolipoyl acyltransferase (EC 2.3.1.12), a branched-chain α-ketoacid dehydrogenase E2 subunit (BCE2), catalyzes the transfer of the acyl group from the lipoyl moiety to coenzyme A. However, the role of BCE2 responding to biotic stress in plant is not clear. In this study, we cloned and characterized a BCE2 gene from potato, namely StBCE2, which was previously suggested to be involved in Phytophthora infestans-potato interaction. We found that the expression of StBCE2 was strongly induced by both P. infestans isolate HB09-14-2 and salicylic acid. Besides, when the homolog of StBCE2 in Nicotiana benthamiana named NbBCE2 was silenced, plants showed increased susceptibility to P. infestans and reduced accumulation of hydrogen peroxide (H2O2). Furthermore, we found that a marker gene NbrbohB involved in the production of reactive oxygen species, was also suppressed in NbBCE2-silenced plants. However, silencing of NbBCE2 had no significant effect on the hypersensitive responses trigged by INF1, R3a-AVR3a(KI) pair or Rpi-vnt1.1-AVR-vnt1.1 pair. Our results suggest that BCE2 is associated with the basal resistance to P. infestans by regulating H2O2 production.

KEYWORDS:

Dihydrolipoyl acyltransferase; Hydrogen peroxide; Nicotiana benthamiana; Phytophthora infestans; Potato

PMID:
24913048
DOI:
10.1016/j.jplph.2014.02.011
[Indexed for MEDLINE]

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