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FEBS Lett. 2014 Aug 25;588(17):2936-43. doi: 10.1016/j.febslet.2014.05.044. Epub 2014 Jun 6.

Characterization of the contractile P2Y14 receptor in mouse coronary and cerebral arteries.

Author information

1
Department of Clinical Experimental Research, Glostrup Research Institute, Copenhagen University Hospital, Nordre Ringvej 59, 2600 Glostrup, Denmark. Electronic address: kristian.agmund.haanes@regionh.dk.
2
Department of Clinical Experimental Research, Glostrup Research Institute, Copenhagen University Hospital, Nordre Ringvej 59, 2600 Glostrup, Denmark.

Abstract

Extracellular UDP-glucose can activate the purinergic P2Y14 receptor. The aim of the present study was to examine the physiological importance of P2Y14 receptors in the vasculature. The data presented herein show that UDP-glucose causes contraction in mouse coronary and basilar arteries. The EC50 values and immunohistochemistry illustrated the strongest P2Y14 receptor expression in the basilar artery. In the presence of pertussis toxin, UDP-glucose inhibited contraction in coronary arteries and in the basilar artery it surprisingly caused relaxation. After organ culture of the coronary artery, the EC50 value decreased and an increased staining for the P2Y14 receptor was observed, showing receptor plasticity.

KEYWORDS:

Basilar artery; Coronary artery; P2Y14; P2Y2 KO; Purinergic receptors; Receptor upregulation

PMID:
24911208
DOI:
10.1016/j.febslet.2014.05.044
[Indexed for MEDLINE]
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