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J Dent (Tehran). 2014 Mar;11(2):225-32. Epub 2014 Mar 31.

Pomegranate juice (punica granatum): a new storage medium for avulsed teeth.

Author information

1
Assistant Professor, Department of pediatric Dentistry, Dental school, Shahed University, Tehran, Iran.
2
Dentist, Department of pediatric Dentistry, Dental school, Shahed University, Tehran, Iran.
3
Assistant Professor, Department of Pharmacology and Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
4
PhD Student, Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
5
General Technician, Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
6
Dentist, Dental Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
7
Proffesor, Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

Abstract

OBJECTIVE:

There is evidence indicating that pomegranate juice contains many of the essential properties necessary to retain cell viability and cell proliferation. These properties indicate that pomegranate juice is a suitable storage medium for avulsed teeth. However, this idea has not yet been tested. In this study, the capacity of pomegranate juice (PJ) as a storage medium for retaining avulsed teeth was evaluated.

MATERIALS AND METHODS:

PDL fibroblasts were obtained from healthy human premolars and cultured in Dulbecco's Modified Eagle's Medium (DMEM). Cultured cells were subjected to different concentrations of pomegranate juice (PJ), 1% Hank's balanced salt solution (HBSS) and tap water for 1, 3, 6 and 24 hours. PDL cell viability was assessed by the neutral red uptake assay.

RESULTS:

The results indicated that 7.5% PJ was the most effective solution for maintaining PDL cell viability amongst all the experimental solution's and time intervals (P<0.05). The results also showed that 1% PJ was as effective as HBSS for maintaining PDL cell viability. The amount of cell viability increased with increasing concentration of PJ at all time intervals (P<0.001). This effect is suggestive of the proliferative potential of PJ solution.

CONCLUSION:

In conclusion, PJ can be recommended as a suitable transport medium for avulsed teeth.

KEYWORDS:

Cell Culture; Neutral Red; Periodontal Ligament; Punica Granatum; Tooth Avulsion

PMID:
24910699
PMCID:
PMC4043555

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