Format

Send to

Choose Destination
Cell. 2014 Jun 19;157(7):1698-711. doi: 10.1016/j.cell.2014.05.018. Epub 2014 Jun 5.

RNA clamping by Vasa assembles a piRNA amplifier complex on transposon transcripts.

Author information

1
European Molecular Biology Laboratory, Grenoble Outstation, University Grenoble Alpes-EMBL-CNRS, 71 avenue des Martyrs, 38042, France; Unit for Virus Host-Cell Interactions, University Grenoble Alpes-EMBL-CNRS, 71 avenue des Martyrs, 38042, France.
2
European Molecular Biology Laboratory, Grenoble Outstation, University Grenoble Alpes-EMBL-CNRS, 71 avenue des Martyrs, 38042, France; Unit for Virus Host-Cell Interactions, University Grenoble Alpes-EMBL-CNRS, 71 avenue des Martyrs, 38042, France; Cell Biology and Biophysics Unit, EMBL, 69117 Heidelberg, Germany.
3
Laboratoire Biologie à Grande Echelle, IRTSV, CEA, 38054 Grenoble, France.
4
Department of Oncological Sciences, Icahn School of Medicine at Sinai, New York, NY 10029, USA.
5
Cell Biology and Biophysics Unit, EMBL, 69117 Heidelberg, Germany.
6
Developmental Biology Unit, EMBL, 69117 Heidelberg, Germany.
7
European Molecular Biology Laboratory, Grenoble Outstation, University Grenoble Alpes-EMBL-CNRS, 71 avenue des Martyrs, 38042, France; Unit for Virus Host-Cell Interactions, University Grenoble Alpes-EMBL-CNRS, 71 avenue des Martyrs, 38042, France. Electronic address: pillai@embl.fr.

Abstract

Germline-specific Piwi-interacting RNAs (piRNAs) protect animal genomes against transposons and are essential for fertility. piRNAs targeting active transposons are amplified by the ping-pong cycle, which couples Piwi endonucleolytic slicing of target RNAs to biogenesis of new piRNAs. Here, we describe the identification of a transient Amplifier complex that mediates biogenesis of secondary piRNAs in insect cells. Amplifier is nucleated by the DEAD box RNA helicase Vasa and contains the two Piwi proteins participating in the ping-pong loop, the Tudor protein Qin/Kumo and antisense piRNA guides. These components assemble on the surface of Vasa's helicase domain, which functions as an RNA clamp to anchor Amplifier onto transposon transcripts. We show that ATP-dependent RNP remodeling by Vasa facilitates transfer of 5' sliced piRNA precursors between ping-pong partners, and loss of this activity causes sterility in Drosophila. Our results reveal the molecular basis for the small RNA amplification that confers adaptive immunity against transposons.

PMID:
24910301
DOI:
10.1016/j.cell.2014.05.018
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center