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Clin Chim Acta. 2014 Sep 25;436:207-16. doi: 10.1016/j.cca.2014.05.022. Epub 2014 Jun 5.

Biomarker identification and pathway analysis by serum metabolomics of childhood acute lymphoblastic leukemia.

Author information

1
Department of Pediatrics, The 2nd Affiliated Hospital of Harbin Medical University, Xuefu Road 246, Nangang District, Harbin 150001, China.
2
Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical University, Baojian Road 157, Nangang District, Harbin 150081, China.
3
Department of Pediatrics, The 2nd Affiliated Hospital of Harbin Medical University, Xuefu Road 246, Nangang District, Harbin 150001, China. Electronic address: renlihong1@126.com.

Abstract

BACKGROUND:

Acute lymphoblastic leukemia (ALL) is a common hematological malignant neoplasm that typically affects children. Although intense chemotherapeutic regimens have been useful to combat the disease, approximately 20% of patients will relapse despite treatment. Diagnosing ALL requires bone marrow puncture procedure, which many parents do not consent to for it is invasive. Additionally, metabolic alterations associated with the disease are unclear.

METHODS:

Metabolic alterations associated with ALL were investigated by performing serum metabolomics based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry and multivariate statistical analysis. Ingenuity Pathways Analysis (IPA) was also performed.

RESULTS:

Thirty metabolites (17 detected in positive mode and 13 in negative mode) were differentially expressed between patients with ALL and control patients; these metabolites were selected as potential biomarkers. Based on IPA analysis, glycerophospholipid metabolism is deregulated in patients with ALL and may represent an underlying metabolic pathway associated with disease progression.

CONCLUSIONS:

Metabolomics can be used to analyze the metabolic activity of ALL patients compared to healthy controls. The data we provide here suggest that glycerophospholipid metabolism may be a key mechanism underlying disease progression and development.

KEYWORDS:

Acute lymphoblastic leukemia; Biomarkers; Metabolomics; Pathway analysis; UPLC/Q-TOF

PMID:
24909874
DOI:
10.1016/j.cca.2014.05.022
[Indexed for MEDLINE]

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