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Oral Surg Oral Med Oral Pathol Oral Radiol. 2014 Jul;118(1):117-125.e1. doi: 10.1016/j.oooo.2014.03.018. Epub 2014 Apr 12.

Next-generation sequencing analysis for detecting human papillomavirus in oral verrucous carcinoma.

Author information

1
Leeds Institute of Cancer and Pathology, University of Leeds, Leeds, United Kingdom.
2
Department of Oncology, Oral Medicine and Oral Oncology Unit, University of Turin, Turin, Italy.
3
Department of Medical Sciences, Pathology Unit, University of Turin, Turin, Italy.
4
Department of Pathology and Laboratory Medicine, National Guard Hospitals, Riyadh, Saudi Arabia.
5
Department of Histopathology, Queen Victoria Hospital, East Grinstead, United Kingdom.
6
Department of Histopathology, St James's University Hospital and Leeds Dental Institute, University of Leeds, Leeds, United Kingdom.
7
Department of Histopathology, St James's University Hospital and Leeds Dental Institute, University of Leeds, Leeds, United Kingdom. Electronic address: a.s.high@leeds.ac.uk.

Abstract

OBJECTIVE:

The etiology of oral verrucous carcinoma is unknown, and human papillomavirus 'involvement' remains contentious. The uncertainty can be attributed to varied detection procedures and difficulties in defining 'gold-standard' histologic criteria for diagnosing 'verrucous' lesions. Their paucity also hampers investigation. We aimed to analyze oral verrucous lesions for human papillomavirus (HPV) subtype genomes.

STUDY DESIGN:

We used next-generation sequencing for the detection of papillomavirus sequences, identifying subtypes and computing viral loads. We identified a total of 78 oral verrucous cases (62 carcinomas and 16 hyperplasias). DNA was extracted from all and sequenced at a coverage between 2.5% and 13%.

RESULTS:

An HPV-16 sequence was detected in 1 carcinoma and 1 hyperplasia, and an HPV-2 sequence was detected in 1 carcinoma out of the 78 cases, with viral loads of 2.24, 8.16, and 0.33 viral genomes per cell, respectively.

CONCLUSIONS:

Our results indicate no conclusive human papillomavirus involvement in oral verrucous carcinoma or hyperplasia.

PMID:
24908602
DOI:
10.1016/j.oooo.2014.03.018
[Indexed for MEDLINE]
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