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Appl Environ Microbiol. 2014 Aug;80(16):4920-9. doi: 10.1128/AEM.00957-14. Epub 2014 Jun 6.

Vertebrate decomposition is accelerated by soil microbes.

Author information

1
Cooperative Institute for Research in Environmental Sciences, University of Colorado, Boulder, Colorado, USA.
2
BioFrontiers Institute, University of Colorado, Boulder, Colorado, USA.
3
BioFrontiers Institute, University of Colorado, Boulder, Colorado, USA Department of Ecology and Evolutionary Biology, University of Colorado, Boulder, Colorado, USA.
4
Laboratory of Forensic Taphonomy, Forensic Sciences Unit, Chaminade University of Honolulu, Honolulu, Hawaii, USA.
5
BioFrontiers Institute, University of Colorado, Boulder, Colorado, USA Howard Hughes Medical Institute, Boulder, Colorado, USA Department of Chemistry and Biochemistry and Department of Computer Science, University of Colorado, Boulder, Colorado, USA rob.knight@colorado.edu.

Abstract

Carrion decomposition is an ecologically important natural phenomenon influenced by a complex set of factors, including temperature, moisture, and the activity of microorganisms, invertebrates, and scavengers. The role of soil microbes as decomposers in this process is essential but not well understood and represents a knowledge gap in carrion ecology. To better define the role and sources of microbes in carrion decomposition, lab-reared mice were decomposed on either (i) soil with an intact microbial community or (ii) soil that was sterilized. We characterized the microbial community (16S rRNA gene for bacteria and archaea, and the 18S rRNA gene for fungi and microbial eukaryotes) for three body sites along with the underlying soil (i.e., gravesoils) at time intervals coinciding with visible changes in carrion morphology. Our results indicate that mice placed on soil with intact microbial communities reach advanced stages of decomposition 2 to 3 times faster than those placed on sterile soil. Microbial communities associated with skin and gravesoils of carrion in stages of active and advanced decay were significantly different between soil types (sterile versus untreated), suggesting that substrates on which carrion decompose may partially determine the microbial decomposer community. However, the source of the decomposer community (soil- versus carcass-associated microbes) was not clear in our data set, suggesting that greater sequencing depth needs to be employed to identify the origin of the decomposer communities in carrion decomposition. Overall, our data show that soil microbial communities have a significant impact on the rate at which carrion decomposes and have important implications for understanding carrion ecology.

PMID:
24907317
PMCID:
PMC4135757
DOI:
10.1128/AEM.00957-14
[Indexed for MEDLINE]
Free PMC Article
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