Abstract
In cultured fibroblasts, urokinase stimulated expression of MMP-9 and generation of ROS, while antioxidant ebselen abolished the stimulating effect of urokinase on MMP-9 expression. sTNF-α produced similar and more pronounced stimulating effect. The data showed that urokinase could regulate MMP-9 expression via ROS generation in fibroblasts, which can play an important role in stimulation of their migration and development of constrictor (negative) vascular remodeling due to thickening of the adventitia.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antioxidants / pharmacology
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Azoles / pharmacology
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Fibroblasts / cytology
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Fibroblasts / drug effects*
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Fibroblasts / metabolism
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Gene Expression / drug effects
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Isoindoles
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Matrix Metalloproteinase 9 / biosynthesis*
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Matrix Metalloproteinase 9 / genetics
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Mice
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NIH 3T3 Cells
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Organoselenium Compounds / pharmacology
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Reactive Oxygen Species / agonists
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Reactive Oxygen Species / antagonists & inhibitors
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Reactive Oxygen Species / metabolism*
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Tumor Necrosis Factor-alpha / pharmacology
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Urokinase-Type Plasminogen Activator / pharmacology*
Substances
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Antioxidants
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Azoles
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Isoindoles
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Organoselenium Compounds
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Reactive Oxygen Species
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Tumor Necrosis Factor-alpha
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ebselen
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Urokinase-Type Plasminogen Activator
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Matrix Metalloproteinase 9