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Carbohydr Polym. 2014 Sep 22;110:405-14. doi: 10.1016/j.carbpol.2014.04.044. Epub 2014 Apr 21.

Chain conformation and immunomodulatory activity of a hyperbranched polysaccharide from Cordyceps sinensis.

Author information

1
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.
2
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China. Electronic address: zhaojing.cpu@163.com.
3
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China. Electronic address: lishaoping@hotmail.com.

Abstract

A polysaccharide, named as cordysinan, extracted from natural Cordyceps sinensis, was identified as a hyperbranched heteropolysaccharide from the results of FT-IR, GC-MS, and carbohydrate analysis by carbohydrate gel electrophoresis analysis, as well as the degree of branching of cordysinan was 43.3%. The solution properties of cordysinan were investigated by using size exclusion chromatography coupled with multi-angle laser light scattering and triple detector array, respectively. The molecular weights, the radius of gyration and the intrinsic viscosity of cordysinan were determined as 22.45±0.26 kDa and 22.37 kDa, 15.4±2.4 nm and 1.41 mL/g, respectively. By applying the polymer solution theory, the exponent (ν and α) values of <S2>g1/2=kMwv and [η]=kMwα were calculated as 0.28 and 0.42, respectively, which firstly revealed that cordysinan existed as a globular shape in 0.9% NaCl aqueous solution. Moreover, the results showed that cordysinan could obviously stimulate macrophages functions.

KEYWORDS:

Chain conformation; Cordyceps sinensis; Immunomodulatory activity; Laser light scattering; Molecular mass; Polysaccharide

PMID:
24906773
DOI:
10.1016/j.carbpol.2014.04.044
[Indexed for MEDLINE]

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